Figure 2:

(A) Schematic of co-culture assembly using magnetic levitation. 3D cultures of VECs (yellow) and VICs (green) were separately levitated for 4 hours. A Teflon pen was used to then sequentially assemble the co-culture together, VECs then VICs. The co-culture is submerged in medium, still attached to the Teflon pen, for 4 hours. The co-culture is then detached off the Teflon and levitated for 3 days in VEC medium. (B) Schematic of the co-culture, within which the VEC layer sits atop the VIC layer. (C) A resulting VIC-VEC culture after 3 days of levitation as seen from above the plate. Scale bar = 5 mm. (D) The planar size of the co-culture over 3 days (n=9). The co-culture starts close to the size of the Teflon pen used to assemble the co-culture. By 12 hours, the co-culture significantly shrinks in size and maintains that size over the next 60 hours. *: p<0.05 versus the rest.