Extended Data Figure 7. Loss of Irg1 enhances the efficacy of anti-PD-1 immune checkpoint blockade.

(A) Depletion of CD8⁺ T cells restored D4M3A tumor growth in Irg1^−/− mice (n=7 mice). P value were calculated by two-sided Tukey’s multiple comparisons test.

(B) Spider plot of D4M3A syngeneic tumors grown in WT and Irg1^−/− mice and treated with anti-PD-1 or isotype control IgG (n=6 mice).

(C-F) Expression of IL6 (C), TGFβ1(D), CXCL10 (E) and IL10 (F) in D4M3A tumor tissues from WT and Irg1^−/− mice that were treated with anti-PD-1 or isotype control IgG (n=5 biologically independent samples).

For all panels, data are represented as mean ± SEM.