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. Author manuscript; available in PMC: 2024 Oct 18.
Published in final edited form as: Neuron. 2023 Oct 18;111(20):3307–3320.e5. doi: 10.1016/j.neuron.2023.09.024

Figure 1: MAPseq of macaque BLA.

Figure 1:

A) Left: schematic of injection approach. Right: actual injection sites plotted on coronal atlas sections (interaural distance in mm). In the anterior section (19.5 mm), injections were made in only one vertical track per animal; in the posterior section (18 mm), injections were made in two tracks per animal. Hemispheres 1 and 2 (red and blue, respectively) came from one animal, while hemispheres 3 and 4 came from the other (yellow and green, respectively). B) Example coronal sections showing dissection targets in striatum as well as frontal and temporal lobes. Ento, entorhinal; HC, hippocampus (not shown); AI, anterior insula cortex; mOFC, medial orbitofrontal cortex; lOFC, lateral orbitofrontal cortex; vlPFC, ventrolateral prefrontal cortex; scACC, subcallosal anterior cingulate cortex; pgACC, perigenual ACC; dACC, dorsal ACC; PMd, dorsal premotor cortex; NAcc, nucleus accumbens; MD, mediodorsal thalamus (also not shown). Anterior-posterior levels are in mm relative to the interaural plane. C) Distribution of barcode counts relative to max found in injection site across non-injection site amygdala samples (blue), all target structures (green; OLS regression vs amygdala: t(2) = 46.43, p < 0.0001), and control sites in cerebellum (red vs targets: t(2) = 4.77, p < 0.001). Significantly more barcode was expressed within the amygdala than distal target sites, and significantly more barcode was expressed in target sites than control tissue from cerebellum, to which the amygdala has a very low probability of projecting. D) Proportion of barcodes in each of the target and control areas; dashed line indicates the proportion of barcode found in control sites. E) Proportion of unique barcodes in no targets outside of amygdala (green), one (orange), two (blue), or more than two targets outside of amygdala (red). F) Normalized proportion of barcodes across all the potential target areas and control areas for 10 unique barcodes (colored lines). G) Conditional probability that a barcode in area A is also found in area B. Note that the order of indexing here alters the probability such that P(A∣B) can be greater than P(B∣A). See also Supplemental Figures 1-5.