Table 2.
Source of reagents
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER | DILUTION |
|---|---|---|---|
| CD3 UCHT1 BV570 | BioLegend | Cat#300436 | 1:50 |
| CD3 SP34-2 V500 | BD Biosciences | Cat#560770 | 1:20 |
| CD4 SK3 BUV661 | BD Biosciences | Cat#612962 | 1:20 |
| CD8a RPA-T8 BUV496 | BD Biosciences | Cat#612942 | 1:50 |
| CD8a RPA-T8 PerCP/Cy5.5 | BioLegend | Cat#301032 | 1:50 |
| CD27 L128 BUV395 | BD Biosciences | Cat#563815 | 1:50 |
| CD28 CD28.2 BV785 | BioLegend | Cat#302950 | 1:50 |
| CD38 HB7 PerCP-eFluor 710 | Invitrogen | Cat#46-0388-42 | 1:15 |
| CD45RA HI100 BUV805 | BD Biosciences | Cat#742020 | 1:50 |
| CD45RA HI100 BV510 | BioLegend | Cat#304142 | 1:50 |
| CD62L DREG-56 FITC | BioLegend | Cat#304804 | 1:20 |
| CD62L DREG-56 PE/Cy7 | BioLegend | Cat#304822 | 1:50 |
| CD69 FN50 BV650 | BioLegend | Cat#310934 | 1:15 |
| CD95 DX2 PE/Cy5 | BioLegend | Cat#305610 | 1:100 |
| CD95 DX2 PE/Dazzle 594 | BioLegend | Cat#305634 | 1:25 |
| CD127 A019D5 BV711 | BioLegend | Cat#351328 | 1:50 |
| CD137 4B4-1 BV605 | BD Biosciences | Cat#745256 | 1:20 |
| Granzyme B QA16A02 PE/Cy7 | BioLegend | Cat#372214 | 1:20 |
| HLA-A2 BB7.2 FITC | BD BBiosciences | Cat#343304 | 1:50 |
| HLA-DR G46-6 BUV737 | BD Biosciences | Cat#748339 | 1:15 |
| Perforin dG9 PE/Dazzle 594 | BioLegend | Cat#308132 | 1:100 |
| Hashtag 1 Antibody | BioLegend | Cat#394601 | 1:04 |
| Hashtag 2 Antibody | BioLegend | Cat#394603 | 1:04 |
| Hashtag 3 Antibody | BioLegend | Cat#394605 | 1:04 |
| Hashtag 4 Antibody | BioLegend | Cat#394607 | 1:04 |
| Hashtag 5 Antibody | BioLegend | Cat#394609 | 1:04 |
| Hashtag 6 Antibody | BioLegend | Cat#394611 | 1:04 |
| Hashtag 7 Antibody | BioLegend | Cat#394613 | 1:04 |
| Hashtag 8 Antibody | BioLegend | Cat#394615 | 1:04 |
| ALNTPKDHI HLA-A*02:01 Alexa Fluor 680 | NIH Tetramer Core | N/A | 1:100 |
| GMSRIGMEV HLA-A*02:01 PE | NIH Tetramer Core | N/A | 1:100 |
| ILLNKHIDA HLA-A*02:01 Alexa Fluor 680 | NIH Tetramer Core | N/A | 1:100 |
| ILLNKHIDA HLA-A*02:01 BV421 | NIH Tetramer Core | N/A | 1:100 |
| LALLLLDRL HLA-A*02:01 APC | NIH Tetramer Core | N/A | 1:500 |
| LLLDRLNQL HLA-A*02:01 APC | NIH Tetramer Core | N/A | 1:100 |
| LLLDRLNQL HLA-A*02:01 BV421 | NIH Tetramer Core | N/A | 1:100 |
| LQLPQGTTL HLA-A*02:01 PE | NIH Tetramer Core | N/A | 1:100 |
| Fixable Viability Stain 780 | BD Biosciences | Cat#565388 | 1:10 |
| LEGEND MAX™ SARS-CoV-2 | BioLegend | Cat#448107 | |
| LEGEND MAX™ SARS-CoV-2 Spike | BioLegend | Cat#447807 | |
| Ficoll-Paque PLUS | Cytiva | Cat#17144003 | |
| EasySep™ Direct Human CD8 + T Cell Isolation Kit | STEMCELL Technologies | Cat#19663 | |
| Brilliant Stain Buffer | BD Biosciences | Cat#563794 | |
| Perm/Wash Buffer | BD Biosciences | Cat#554723 | |
| Opti-MEM™ I Reduced Serum Medium | Thermo Fisher Scientific | Cat#11058021 | |
| DMEM, Dulbecco’s Modified Eagle Medium | Thermo Fisher Scientific | Cat#11965092 | |
| FuGENE® HD Transfection Reagent | Promega | Cat#E2311 | |
| Chromium Next GEM Single Cell 5’ Reagent Kits v2 (Dual Index) | 10X Genomics |