Table 4.
Description of genetic profile of keratinocyte cells after cellular target detection.
| Keratincoyte cells | ||||||
|---|---|---|---|---|---|---|
| Usability of profile | Degradation index | Number of missing loci | Amelogenin | Q/S | ||
| Control | 2/2 |
1.2 1 |
0/22 0/22 |
X–Y | Q/S | |
| Dapi | Con A | 2/2 |
0.8 1 |
0/22 0/22 |
X–Y | Q/S |
| PNA | 2/2 |
1.2 1.5 |
0/22 0/22 |
X–Y | Q/S | |
| SNA | 2/2 |
1.2 1.2 |
0/22 0/22 |
X–Y | Q/S | |
| UEA | 2/2 |
1 1 |
0/22 0/22 |
X–Y | Q/S | |
| Lam | 2/2 |
0.8 1.3 |
0/22 0/22 |
X–Y | Q/S | |
| K10 | 2/2 |
1.1 0.8 |
0/22 0/22 |
X–Y | Q/S | |
| Hoechst | Con A | 2/2 |
1.2 1 |
0/22 0/22 |
X–Y | Q/S |
| PNA | 2/2 |
1.1 1.3 |
0/22 0/22 |
X–Y | Q/S | |
| SNA | 2/2 |
0.9 1 |
0/22 0/22 |
X–Y | Q/S | |
| UEA | 2/2 |
1.2 2 |
0/22 0/22 |
X–Y | Q/S | |
| Lam | 2/2 |
1.1 0.9 |
0/22 0/22 |
X–Y | Q/S | |
| K10 | 2/2 |
1 0.9 |
0/22 0/22 |
X–Y | Q/S | |
| Anti-H2B | Con A | 2/2 |
1 1.1 |
0/22 0/22 |
X–Y | Q/S |
| PNA | 2/2 |
0.9 1 |
0/22 0/22 |
X–Y | Q/S | |
| SNA | 2/2 |
1.1 0.9 |
0/22 0/22 |
X–Y | Q/S | |
| UEA | 2/2 |
0.8 1.3 |
0/22 0/22 |
X–Y | Q/S | |
| Lam | 2/2 |
1.2 1 |
0/22 0/22 |
X–Y | Q/S | |
| K10 | 2/2 |
1.5 1 |
0/22 0/22 |
X–Y | Q/S | |
The height of the peaks was measured in rfu (relative fluorescence unit): it was proportional to the quantity of PCR products detected. A profile was usable if the number of complete Short Tandem Repeat (STR) markers was greater than 5, according to the requirements of the French database Fichier National Automatisé Empreintes Génétiques. A profile was complete if all STR markers (22 STR) were amplified and present. Degradation index indicated the quality of the DNA (value close to 0: complete degradation; value close to 1: no degradation). Human gender determination was performed by analyzing the amelogenin gene which is common to both X and Y chromosomes. If the two quality sensors (Q and S) were visible on the electrophoregram, it meant that the PCR had been successful and there was no inhibition. 2/2 indicates the number of usable profiles per replicate. Results are representative of at least two independent experiments.