Table 5.
Description of genetic profile of fingermarks after cellular target detection.
| Fingermark samples | ||||||
|---|---|---|---|---|---|---|
| Usability of profile | Degradation index | Number of missing loci | Amelogenin | Q/S | ||
| Control | 3/3 |
1.1 0.8 0.7 |
5/22 4/22 4/22 |
X-X | Q/S | |
| Dapi | Con A | 2/3 |
0.8 0.9 1.8 |
5/22 5/22 19/22 |
X-X | Q/S |
| PNA | 3/3 |
1.2 0.8 0.8 |
17/22 17/22 10/22 |
X-X | Q/S | |
| SNA | 3/3 |
0.8 0.8 0.9 |
13/22 17/22 16/22 |
X-X | Q/S | |
| UEA | 3/3 |
1.5 1 0.8 |
14/22 16/22 10/22 |
X-X | Q/S | |
| Lam | 3/3 |
0.7 0.7 0.8 |
14/22 12/22 16/22 |
X-X | Q/S | |
| K10 | 2/3 |
0.9 0.8 0.7 |
15/22 14/22 20/22 |
X-X | Q/S | |
| Hoechst | Con A | 1/3 |
0.8 0.7 0.9 |
20/22 1/22 18/22 |
X-X | Q/S |
| PNA | 3/3 |
0.7 0.9 0.7 |
16/22 2/22 2/22 |
X-X | Q/S | |
| SNA | 2/3 |
0.7 0.7 0.7 |
21/22 9/22 3/22 |
X-X | Q/S | |
| UEA | 2/3 |
0.8 0.7 0.7 |
14/22 5/22 19/22 |
X-X | Q/S | |
| Lam | 3/3 |
0.7 0.7 0.7 |
17/22 11/22 10/22 |
X-X | Q/S | |
| K10 | 1/3 |
0.9 0.7 0.7 |
18/22 9/22 22/22 |
X-X | Q/S | |
| Anti-H2B | Con A | 3/3 |
0.8 0.6 1 |
16/22 15/22 12/22 |
X-X | Q/S |
| PNA | 2/3 |
0.7 0.8 1 |
1/22 18/22 11/22 |
X-X | Q/S | |
| SNA | 3/3 |
0.7 0.8 1.2 |
16/22 9/22 16/22 |
X-X | Q/S | |
| UEA | 1/3 |
0.7 0.9 0.7 |
18/22 19/22 3/22 |
X-X | Q/S | |
| Lam | 3/3 |
0.7 0.7 1 |
2/22 8/2 17/22 |
X-X | Q/S | |
| K10 | 0/3 |
0.7 0.7 0.8 |
20/22 18/22 18/22 |
X-X | Q/S | |
The height of the peaks was measured in rfu (relative fluorescence unit): it was proportional to the quantity of PCR products detected. A profile was usable if the number of complete Short Tandem Repeat (STR) markers was greater than 5, according to the requirements of the French database Fichier National Automatisé Empreintes Génétiques. A profile was complete if all STR markers (22 STR) were amplified and present. Degradation index indicated the quality of the DNA (value close to 0: complete degradation; value close to 1: no degradation). Human gender determination was performed by analyzing the amelogenin gene which is common to both X and Y chromosomes. If the two quality sensors (Q and S) were visible on the electrophoregram, it means that the PCR was successful and there was no inhibition. 3/3 indicates the number of usable profiles per replicate. Results are representative of at least three independent experiments.