Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 23;14:6721. doi: 10.1038/s41467-023-42564-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Body weights (BW) of floxed and OPA1-LKO mice after AAV-Cre injection. The week 0 is the day of injection. b Weekly changes of body weights normalized to the initial weight are plotted. n = 13 (flox) and 17 (KO) animals for 0–5 weeks; n = 12 (flox) and 16 (KO) animals for 6–8 weeks; n = 6 (flox) and 8 (KO) animals for week 9; n = 5 (flox) and 7 (KO) animals for 10−14 weeks. Data are presented as mean values +/- SD. Two-way ANOVA with Sidak’s multiple comparisons. ***p = 0.0005, ****p < 0.0001. c Blood glucose measurements in non-fasted and fasted conditions. n = 5 animals per group for nonfasted; n = 10 (flox) and 7 (KO) animals for fasted. Data are presented as mean values +/- SD. Unpaired t test. d Glucose tolerance test (GTT) showing faster glucose clearance in OPA1-LKO mice. n = 11 (flox) and 7 (KO) animals. Data are presented as mean values +/- SD. Two-way ANOVA with Sidak’s multiple comparisons. ***p = 0.0001. Area under curve (AUC) shows an improved glucose clearance by OPA1 KO. Data are presented as mean values +/- SD. Unpaired t test with Welch’s correction. **p = 0.0024. e–j Assessment of metabolic parameters. n = 4 for floxed and OPA1-LKO mice. Hourly average is plotted for 72 hours. Shaded periods denote the dark cycle. Error bars are omitted. Bar graphs are the 3-day hourly average for light and dark cycles as well as total. n = 4 animals per group over 10 measures for light and dark cycles and 20 measures for total. Data are presented as mean values +/- SD. Multiple unpaired t test. The p values for light, dark, and total, respectively, are: (e) VO₂, p < 0.000001, p < 0.000034, and p < 0.000001; (f) VCO₂, p < 0.000001, p = 0.000013, and p < 0.000001; (g) respiratory exchange ratio; p < 0.000001, p = 0.000019, and p < 0.00001; (h) energy expenditure, p = 0.000032, p = 0.004018, and p = 0.000340; (i) food consumption, p = 0.000209, p = 0.546617, and p = 0.217867; (j) total activity, p = 0.011132, p = 0.317263, and p = 0.803317. Source data are provided as a Source Data file.