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. 2023 Oct 23;14:6721. doi: 10.1038/s41467-023-42564-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a H&E staining of liver sections from control and OPA1-KO mice with and without APAP. Representative images from at least 7 animals per group. The results were consistent. b, c OPA1 KO mitigates the ALT increase and GSH depletion in APAP treatment. n = 7 (b, ALT), 5 (saline in c) and 6 (APAP in c) animals. Data are presented as mean values +/- SD. Two-way ANOVA with Turkey’s multiple comparisons. ***p = 0.0003; **p = 0.0054 (b). ****p < 0.0001; ***p = 0.0007; **p = 0.0046; *p = 0.0102 (c). d, e Immunoblots for p-JNK and LC3 in floxed and OPA1-KO livers with and without APAP. n = 5 animals per group. Data are presented as mean values +/- SD. Two-way ANOVA with Turkey’s multiple comparisons. ****p < 0.0001 (OPA1); **p = 0.0066 (p-JNK); *p = 0.0291 (p-JNK), 0.0281 (LC3), and 0.0284 (LC3). f A heatmap of CYPs found in total and mitochondrial proteomes. Arrow: CYP2E1. g CYP2E1 immunoblot. Total protein is shown in Fig. 9i. n = 5 animals per group. Data are presented as mean values +/- SD. Unpaired t test. p < 0.0001. h Quantification of liver APAP-CYS; n = 5 animals per group. Data are presented as mean values +/- SD. Unpaired t test. p = 0.0003. Source data are provided as a Source Data file.