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. 2023 Oct 23;14(10):695. doi: 10.1038/s41419-023-06222-z

Fig. 3. TRIM26 suppresses ferroptosis in glioma cells.

Fig. 3

a Trypan blue staining assays using shCtrl or shTRIM26 T98 and U118 cells treated with ferrostatin-1(100 nM) or liproxstatin-1 (50 nM). Data are represented as mean ± SD (n = 5); student’s t test; **P < 0.01, ***P < 0.001. b Trypan blue staining assays using LN229 and A172 cells expressing vector control or TRIM26 treated with Erastin (20 µM) or RSL3 (5 µM). Data are represented as mean ± SD (n = 5); student t test; **P < 0.01, ***P < 0.001. c–f Relative MDA (c), 4-HNE (d) and ROS levels (e, f) in T98G and U118 cells transfected as indicated. Data are represented as the mean ± SD (n = 3); student’s t-test; **P < 0.01, ***P < 0.001. g–j Luciferase expressing T98G (g) and U118 (i) cells were transduced with indicated plasmids and then injected into mice brains. Representative bioluminescent live (BLI) images of three mice are shown. Kaplan-Meier survival curves of mice intracranially injected with T98G (h) and U118 (j) cells with the indicated modifications (n = 8); log-rank (Mantel-Cox) test, **P < 0.01, ***P < 0.001.