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. 2023 Oct 23;14:6694. doi: 10.1038/s41467-023-42478-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The products of PlsMsmg acyltransferase reactions using 1-hydroxy-2-palmitoyl-G3P as the acceptor substrate and C18:1-CoA or C18:0-CoA as acyl donors were analyzed by LC/MS. a shows the generation of LPA acceptor substrates from 1,2-dipalmitoyl-sn-G3P upon phospholipase A1 digestion. Both sn-1 and sn-2 LPAs are found as a result of the spontaneous (non-enzymatic) transmigration of C16:0 from position sn-2 to position sn-1. b Incubation of this LPA mixture with PlsMsmg in the presence of C18:1-CoA or C18:0-CoA yields PA products harboring C16:0/C18:0 or C16:0/C18:1 acyl chains. c Digestion of these PA products with phospholipase A2 solely yields sn-1 LPA products harboring a C16:0 chain indicating that PlsMsmg transferred C18:0 or C18:1 to the sn-2 position of 1-palmitoyl-2-hydroxy-G3P rather than to the sn-1 position of 1-hydroxy-2-palmitoyl-G3P.