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. 2023 Oct 2;11:1183. Originally published 2022 Oct 17. [Version 2] doi: 10.12688/f1000research.126787.2

Figure 8. Voronoi tessellation of slices.

Figure 8.

Confocal images of the GFP-tagged PNs with superimposed Voronoi polygons (A, D, and G) in 21 DIV slices. Polygons were generated and further elaborated as described in the Methods section and protocols.io. Images in B, E, and H show the initial elaboration of Voronoi polygons; those in C, F, and I show the last step of elaboration with the exclusion of the marginal polygons that are outside the convex hull. It can be seen that polygons are smaller and have a more homogeneous size in single cultured slices from L7-GFP reln (-/-)F1/ mice (D-F), become larger and have less homogeneous sizes in co-cultured slices from L7-GFP reln (-/-)F1/ mice (G-I), whereas slices from L7-GFP reln (+/-)F1/ mice display larger polygons of quite homogeneous sizes. Black dots in A-B, D-E, and G-H are the centers of mass of the PNs. They have been cleared in the subsequent elaboration (C, F, and I) to avoid interference with automated counting. Colorization is solely used for better visualization of polygons. Abbreviations: GFP = green fluorescent protein; PCL = Purkinje cell layer; PNs = Purkinje neurons.