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. 2005 Mar 3;115(4):910–918. doi: 10.1172/JCI22850

Figure 1.

Figure 1

Generation of Pkd1–/– ES cells. (A and B) Genomic organization of 2 targeting vectors. Exons are depicted as filled boxes. The targeting vectors were designed to replace a DNA segment of exons 2–6 by a neomycin-resistance gene cassette (neo) (A) or a hygromycin-resistance gene cassette (hyg) (B). EGFP, gene encoding enhanced GFP. (C and D) Southern blots of genomic DNA derived from ES clones. Purified DNA was digested with EcoRV and bands were detected by a probe, as described in Methods. Fragments corresponding to wild-type (15.1 kb) and targeted (7.7 kb and 8.3 kb) alleles are shown. +/+, wild-type; –/–, Pkd1–/–.