Table 1. Rate Constants k_obs of B. subtilisglmS Ribozyme Cleavage Induced by Compounds (R)-2, (S)-2, (R)-3, (S)-3, Methylene Phosphonate C, Thia-GlcN6P 4, and GlcN6P.

		kobs [min–1]
compound	pKa2	@ 10 μM	@ 200 μM	@ 500 μM	@ 1 mM
GlcN6P	6.2 ± 0.1	0.820 ± 0.04	2.21 ± 0.18	2.49 ± 0.28	n.d.
C	7.5 ± 0.1	n.d.	0.103 ± 0.004	0.332 ± 0.01	0.439 ± 0.05
(R)-2	7.2 ± 0.1	n.d.	0.069 ± 0.004	0.211 ± 0.01	0.270 ± 0.01
(S)-2		n.d.	0.078 ± 0.004	0.157 ± 0.01	0.362 ± 0.03
(R)-3		n.d.	0.049 ± 0.002	0.174 ± 0.01	0.173 ± 0.01
(S)-3	6.3 ± 0.1	n.d.	n.d.	0.091 ± 0.004	0.055 ± 0.003
4		1.09 ± 0.15	2.44 ± 0.12	2.36 ± 0.04	n.d.