Fig. 3.

Experimentally infected sheep exhibited dynamic immune responses over the study time course. Sheep peripheral blood was analyzed for specific cell subsets using flow cytometry and cell specific antibodies. Broader cell populations, as indicated by complete blood counts, show (A) lymphocytes, and (B) neutrophils cell counts. CBC data is shown as BTV-17 infected relative to mock (set to 100 %) for each timepoint. Specific cell populations were identified by flow cytometry (C) CD8+ T cells, (D) CD4+ T cells, the CD8+/CD4+ T cells ratio (E), (F) B cells, (G) and CD14+ monocytes were detected and evaluated. Data is shown as mock infected (green) and BTV-17 infected (red). To account for animal-to-animal variation, all data points were standardized to signal at day 0..