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. 2023 Oct 23;23:1022. doi: 10.1186/s12885-023-11529-3

Table 1.

DNA targets according to Gen Bank and nucleotide sequences of primers and probes

Target gene Primer or probe Sequence (5’→ 3’ ) Product length notes
HPV 52 Forward GACATGTTAATGCAAACAAGCG 103
Reverse TGACGTTACACTTGGGTCAC
Probe CAGAGTGTTGGAGACCCCGACC
HPV 58 Forward GGCATGTGGATTTAAACAAAAGG 121
Reverse TCTCATGGCGTTGTTACAGG
Probe TGGAGACCCCGACGTAGACAAAC
HPV 56 Forward 1 TGCATTGTGACAGAAAAAGACG 73
Forward 2 AAAGCAATTGCMTTGTGACAGA (M = A or C)
Reverse 1 CTCCAGCACCCCAAACATG
Reverse 2 GATGTYTGTCTCCAGCACC (Y = C or T)
Probe CCCGGTCCAACCATGTGCTATTAG
HPV 6 Forward CGGTTYATAAAGCTAAATTGTACG 78 (Y = C or T)
Reverse GGGTAACATGTCTTCCATGCA
Probe AGGGTCGCTGCCTACACTGCTG
HPV 11 Forward GCTTCATAAAACTAAATAACCAGTGG 110
Reverse CAGGAGGCTGCAGGTCTAG
Probe CCAGCAGTGTAAGCAACGACCC
HPV 16 Forward GCAGATCATCAAGAACACGTAG 108
Reverse TAGAGATCAGTTGTCTCTGGTTG
Probe CATGCATGGAGATACACCTACATTGCATG
HPV 18 Forward AGAGGCCAGTGCCATTCG 65
Reverse GTTTCTCTGCGTCGTTGGAG
Probe CTGTCGTGCTCGGTTGCAGC
HPV 33 Forward TTTCGGGTCGTTGGGCA 71
Reverse ACGTCACAGTGCAGTTTCTC
Probe CCTCCAACACGCCGCACA
HPV 45 Forward CAGTACCGAGGGCAGTGTA 68
Reverse TCCCTACGTCTGCGAAGTC
Probe CATGTTGTGACCAGGCACGGC