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. 2023 Oct 23;21:130. doi: 10.1186/s12969-023-00909-5

Table 1.

Primers used for qPCR experiments

Primer name Primer sequence Primer length (nt) Product length (bp) Annealing temperature (℃)
Nrf2 F CTTTAGTCAGCGACAGAAGGAC 22 227 47.8
Nrf2 R AGGCATCTTGTTTGGGAATGTG 22
HMGB1 F GGCGAGCATCCTGGCTTATC 20 86 60.1
HMGB1 R GGCTGCTTGTCATCTGCTG 19
TLR4 F GAAGCTTGAATCCCTGCATAGAGGT 25 249 49.0
TLR4 R AGTTTGAGAGGTGGTGTAAGCC 22
NF-κB F CACGAGGCTCCTTTTCTCAA 20 270 58.3
NF-κB R GGGGTTCAGTTGGTCCATTG 20
β-actin F AGGGAAATCGTGCGTGAC 18 192 58.0
β-actin R CATACCCAAGAAGGAAGGCT 20