Table 1. Parameters’ Description and Values.

parameter	description	value	unit
δRNA	degradation constant for first-order degradation of guide RNA	10.8	hr–1
γi,j	transcription rates of guide RNA/scRNA (competing guide RNAs)	530.1	nM hr–1
Kj	transcription rate due to leaky promoter	5	hr–1
Ki	transcription rate from dCas9 bound promoter (activation)	100	hr–1
δGFP	degradation constant for first-order degradation of GFP RNA	1.176	hr–1
Di,j	concentration of free target DNA binding sites for scRNA (i) or sgRNA (j)	variable	nM
Di,jtotal	total concentration of target DNA binding sites for scRNA (i) or sgRNA (j)	30	nM
dtotal	total quantity of dCas9	100 or 10,000
mtotal	total quantity of MCP-SoxS	100
ki	scRNA (xi), dCas9, and MCP-SoxS complex formation affinity	1
kj	sgRNA (xj) and dCas9 complex formation affinity	variable (1–100,000)
qi	DNA binding affinity of the CRISPRa complex	1
qj	DNA binding affinity of CRISPRi complex	1 or 100,000
xi	concentration of scRNA	variable
xj	concentration of sgRNA	variable