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Northern blot analysis of total RNA from MSSA strains after growth without (−) and with (+) methicillin (0.25 μg/ml). RNA was prepared from cells after 8 h of growth in modified LB broth. Six micrograms of RNA was loaded into each well. After electrophoresis and blotting, the filter was probed for hla mRNA with a peroxidase-labelled 722-nt intragenic hla-specific ClaI fragment.
