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Immunoblot analysis of alpha-toxin production of MRSA strains after growth without (−) and with methicillin (10 and 50 μg/ml). Culture supernatant samples were taken following 18 h of incubation, and 10 μl of each sample was loaded onto the gel. As a control, 1 μg of purified alpha-toxin (Sigma) was used in the control lane.
