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. 2023 Oct 10;4:1261121. doi: 10.3389/fragi.2023.1261121

FIGURE 1.

FIGURE 1

Expression of the X-chromosomal miRNA cluster. Relative expression levels for young (3 months) and old (24 months) male (A) and Female (B) mouse liver determined by Nanostring analysis. Note, all of the miRNAs in this cluster increase in expression by 24 months in wild-type mice. p < 0.001 and 0.02 for the miRNAs in the cluster in males and females, respectively. Normalized expression of the stably expressed liver-specific mir-122 is shown as a control to document that there is not a general increase in expression of all miRNAs in the aged mice. NS, not significant; p = 0.49 and 0.82 for miR-122 in males and females, respectively. Error bars represent standard deviation. (C) Quantitative PCR (qPCR) reveals that calorie restriction attenuates the increase in expression in male mouse liver for several of the miRNAs at 24 months of age as determined by qPCR. (D) The long-lived Ames dwarf genetic background attenuates the increase in miRNA expression for several of the miRNAs in male mouse liver, determined by qPCR. Error bars represent standard error of the mean (S.E.M.). AL, ad libitum fed, CR, calorie restriction. n = 6–9. ***, p < 0.001; **, p < 0.01; *, p < 0.05.