TABLE 3.

“High-confidence drug resistance-conferring mutations” ^a identified in minority variants from specimens with more than one M. tuberculosis strain (N = 183)

Strain ID	pDST profile	Majority drug-resistant variant	Mixed strain population ratio	Minority drug-resistant variant
				Mutation	POS	Nucleotide change	Read depth	AF (%)
17-3391-0083	INH-R, RIF-S	fabG1 -15 c > t	63%:37%	rpoB_H445N	761139	C to A	222	4.1
18-1773-0435	INH-R	S b	81.9%:18.1%	katG_S315T	2155168	C to G	62	11.3
18-1773-0007	S	S	80.4%:19.6%	katG_S315T	2155168	C to G	89	20.2
17-3391-0281	S	S	57.9%:42.1%	pncA_V128G	2288859	A to C	244	6.6
17-3391-0008	S	S	51.7%:48.3%	embB_D354A	4247574	A to C	140	8.6

^a Group 1 (“Assoc W R”) drug-resistant variants, as in 2021 WHO Catalogue (23, 24).

^b No isoniazid-associated resistance mutation detected.