Figure 1.

Effect of acidic pH on the replication of LF82 in macrophages. (A) The pH variation between uninfected macrophages and LF82-infected macrophages was indicated by LysoSensor™ Yellow/Blue. (B) The intracellular replication of LF82 in macrophages and macrophages treated with 30 mM NH₄Cl or 10 µM CQ. (C) Confocal observation of LF82 (red) intracellular replication in macrophages and macrophages treated with 30 mM NH₄Cl or 10 µM CQ at indicated time point. (D) Growth curve of LF82 treated with 30 mM NH₄Cl or 10 µM CQ in LB medium. (E) Growth curve of LF82 treated with 30 mM NH₄Cl or 10 µM CQ in 1640 medium. Data were obtained from three independent experiments and analyzed using Student’s t-test. ***P < 0.001.