Figure 2.

The phenotypic effects of chitosan (CHT), diamino 1 (D1), and diamino 2 (D2) treatment on the disease progression of B. cinerea, 4 days post-inoculation (dpi). The treatments were tested by direct or systemic application and imaged thereafter. Lesion development and progression were noted by the spreading of dark red/black (F_v/F_m, 0-0.5) or yellow/white (ChlIdx, 0-1.8) spots as measured by the false color scales. Healthy leaf tissue was noted as yellow/green (F_v/F_m,0.7-0.9) or green (ChlIdx, 1.9-2.5) by the false color scales. (A) Images displaying the direct effects of treatment on F_v/F_m at 4 dpi. (B) Images displaying the systemic effects of treatment on F_v/F_m at 4 dpi. (C) RGB, F_v/F_m, mAriIdx, and ChlIdx images of direct D1 treatment at 4 dpi and a detailed view of the effects with 1 and 2.5 mg/mL. (D) RGB, F_v/F_m, mAriIdx, and ChlIdx images of systemic D1 treatment at 4 dpi and a detailed view of all concentrations. Leaves were inoculated 24 hours after polymer spraying with two 10 µL droplets of a B. cinerea spore suspension (1 x 10⁶ spores/mL containing 0.01 M glucose and 6.7 mM KH₂PO₄). The images represent the average phenotype of two experiments.