Figure 6.

The temporal accumulation of H₂O₂ in leaf discs treated with diamino 3 (D3) and D3 lower MW fractions (F1-F3) and visualized with DAB staining at 4, 8, 12, 16 and 20 hours post-inoculation (hpi) with B. cinerea. (A) Macroscopic images of DAB staining at 20 hpi displaying the progression of disease symptoms as noted by the appearance of brown spots in the RGB image. (B) Macroscopic image of DAB staining in de-stained leaf discs sprayed with different polymer concentrations and inoculated with a mock solution at 20 hpi. (C) The macroscopic, temporal H₂O₂ accumulation at 4, 8, 12, 16, and 20 hpi. This image correlates with the RGB image in (A). Leaf discs were inoculated with two 10 µL droplets of a B. cinerea spore solution (1 x 10⁶ spores/mL containing 0.01 M glucose and 6.7 mM KH₂PO₄) 24 hours after spraying. The image here represents one of four biological replicates.