Table 4.
The effects of various concentrations of diamino 3 (D3) and the D3 MW fractions (F1-F3) on the average mycelial radial growth and sporulation of B. cinerea, 11 days after incubation.
| Treatment | Concentration (mg/mL) |
Radial Inhibition | Sporulation | ||
|---|---|---|---|---|---|
| Growth area (mm2) ± SD |
PIRG (%) ± SD | Spores/mL ± SD | PIS (%) ± SD | ||
| PDA | 0 | 491 ± 0 | 0 ± 0a | 21 133 ± 17 365 | 0 ± 0a |
| Diamino 3 | 0.5 | 457 ± 48 | 7 ± 7a | 5 500 ± 1 980 | 77 ± 18b |
| 1 | 237 ± 259 | 52 ± 37b | 4 750 ± 4 455 | 86 ± 8b | |
| 2.5 | 49 ± 58 | 90 ± 8c | 5 850 ± 8 132 | 85 ± 21b | |
| 5 | 20 ± 33 | 96 ± 0c | 300 ± 0 | 100 ± 0b | |
| PDA | 0 | 491 ± 0 | 0.00a | 11 900 ± 14 100 | 0a |
| F1 (3.5-5 kDa) | 0.5 | 358 ± 87 | 28.36ab | 8 300 ± 4 600 | 30.25a |
| 1 | 235 ± 93 | 52.23bc | 3 700 ± 4 200 | 68.91ab | |
| 2.5 | 47 ± 38 | 90.49c | 500 ± 0 | 95.80b | |
| PDA | 0 | 491 ± 0 | 0.00a | 11 900 ± 14 100 | 0a |
| F2 (15 kDa) | 0.5 | 373 ± 162 | 23.97ab | 23 500 ± 32 200 | -97.48a |
| 1 | 270 ± 133 | 45.01bc | 2000 ± 3 600 | 83.19b | |
| 2.5 | 0 ± 0 | 100.00c | 100 ± 200 | 99.16b | |
| PDA | 0 | 491 ± 0 | 0.00a | 11 900 ± 14 100 | 0ab |
| F3 (20 kDa) | 0.5 | 455 ± 80 | 7.33a | 5 500 ± 5 500 | 53.78ac |
| 1 | 491 ± 0 | 0.03a | 1 500 ± 900 | 87.39c | |
| 2.5 | 121 ± 75 | 75.42b | 19 100 ± 9 100 | -60.50b | |
PIRG% = percentage inhibition of radial growth (PIRG), and PIS% = percentage inhibition of sporulation (PIS). A negative PIS% indicates growth greater than the control. Means ± SD (standard deviation) followed by the same superscript letter are not significantly different from each other (Kruskal-Wallis test followed by Dunn’s post-hoc test, p< 0.05). The values shown are the average of three experiments for D3 and two experiments for the fractions.