TABLE 1.
Relevant characteristics of the 24 wild-type strains and their transconjugantsa
| Strain no. (designation) | Hospital (city) (yr of isolation) | Hemolysis | Antibiotic resistance markers of wild-type strain | Frequency of conjugative transfer of Gmr marker into JH2-2 | Antibiotic resistance markers of transconjugantsb | Gmr plasmid in transconjugants or wild-type strains |
|---|---|---|---|---|---|---|
| 1 (D362)c | Hotel Dieu (Paris) (1978) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 7 × 10−2 | Cmr Gmr-Kmr | pIP655 |
| 2 (D367) | Hotel Dieu (Paris) (1978) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 2 × 10−1 | Cmr Gmr-Kmr | pIP687 |
| 3 (D366) | Boucicaut (Paris) (1978) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 3 × 10−2 | Cmr Gmr-Kmr | pIP683 |
| 4 (8942) | Broussais (Paris) (1981) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−1 | Cmr Gmr-Kmr | pIP1722 |
| 5 (8943) | Broussais (Paris) (1981) | NH | Cmr Emr Gmr-Kmr | <1 × 10−9 | NA | pIP1723d |
| 6 (D396) | Broussais (Paris) (1983) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 3 × 10−6 | Cmr Gmr-Kmr | pIP1724 |
| 7 (9636) | Broussais (Paris) (1984) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 2 × 10−5 | Gmr-Kmr | pIP1730 |
| 8 (10028) | Broussais (Paris) (1985) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−5 | Gmr-Kmr | pIP1731 |
| 9 (10029) | Broussais (Paris) (1985) | NH | Gmr-Kmr Tcr-Mnr | 3 × 10−6 | Gmr-Kmr | pIP1732 |
| 10 (10035) | Broussais (Paris) (1985) | NH | Emr Gmr-Kmr Tcr-Mnr | <1 × 10−9 | NA | pIP1733d |
| 11 (10042) | Broussais (Paris) (1985) | NH | Gmr-Kmr Tcr-Mnr | <1 × 10−9 | NA | NA |
| 12 (10628) | Broussais (Paris) (1988) | NH | Emr Gmr-Kmr Tcr-Mnr | <1 × 10−9 | NA | NA |
| 13 (10586) | Broussais (Paris) (1988) | β | Emr Gmr-Kmr Tcr-Mnr | 1 × 10−1 | Gmr-Kmr | pIP1734 |
| 14 (10619) | Broussais (Paris) (1988) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−2 | Emr Gmr-Kmr | pIP1735 |
| 15 (10629) | Broussais (Paris) (1988) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 2 × 10−3 | Cmr Emr Gmr-Kmr | pIP1736 |
| 16 (10630) | Broussais (Paris) (1988) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 2 × 10−3 | Cmr Emr Gmr-Kmr | pIP1737 |
| 17 (10282) | Saint-Joseph (Paris) (1986) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | <1 × 10−9 | NA | NA |
| 18 (9850) | Pontchaillou (Rennes) (1984) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | <1 × 10−9 | NA | pIP1725d |
| 19 (9851) | Pontchaillou (Rennes) (1984) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−5 | Gmr-Kmr | pIP1726 |
| 20 (9852) | Pontchaillou (Rennes) (1984) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−5 | Gmr-Kmr | pIP1727 |
| 21 (9853) | Pontchaillou (Rennes) (1984) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−5 | Gmr-Kmr | pIP1728 |
| 22 (9854) | Pontchaillou (Rennes) (1984) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | 1 × 10−5 | Gmr-Kmr | pIP1729 |
| 23 (10703) | (Chateauroux) (1989) | β | Cmr Emr Gmr-Kmr Tcr-Mnr | 2 × 10−2 | Emr Gmr-Kmr | pIP1738 |
| 24 (10907) | (Longjumeaux) (1990) | NH | Cmr Emr Gmr-Kmr Tcr-Mnr | <1 × 10−9 | NA | NA |
a
Abbreviations: β, beta-hemolysis; Gmr-Kmr, high-level gentamicin-kanamycin resistance; NA, not applicable; NH, nonhemolytic.
b
All the transconjugants studied were nonhemolytic.
c
Reference 9 for strains 1 to 3; this study for strains 4 to 24.
d
Plasmid isolated from the wild-type strains.