TABLE 2.

Location of the Gm^r marker and of IS256 on plasmid and cellular DNA

Strain no. (designation)	Gmr plasmid designation	Conjugative transfer (Tra)a	Hybridization with Gmr and IS256 probe (HindIII digestion)				Structure of the element carrying the Gmr marker (Fig. 1)
			Plasmid DNA		Cellular DNA
			Gmr	IS256	Gmr	IS256
1 (D362)	pIP655	Tra+	+	+	+	+	Tn4001-truncated I
2 (D367)	pIP687	Tra+	+	+	+	+	Tn4001-truncated I
3 (D366)	pIP683	Tra+	+	+	+	+	Tn4001-truncated I
4 (8942)	pIP1722	Tra+	+	+	+	+	Tn4001-truncated I
5 (8943)	pIP1723	Tra−	+	+	+	+	Tn4001-truncated I
7 (9636)	pIP1730	Tra+	+	+	+	+	Tn4001-truncated I
8 (10028)	pIP1731	Tra+	+	+	+	+	Tn4001-truncated I
9 (10029)	pIP1732	Tra+	+	+	+	+	Tn4001-truncated I
10 (10035)	pIP1733	Tra−	+	+	+	+	Tn4001-truncated I
13 (10586)b	pIP1734	Tra+	+	+	+	+	Tn4001-truncated I
18 (9850)	pIP1725	Tra−	+	+	+	+	Tn4001-truncated II
19 (9851)	pIP1726	Tra+	+	+	+	+	Tn4001-truncated II
20 (9852)	pIP1727	Tra+	+	+	+	+	Tn4001-truncated II
21 (9853)	pIP1728	Tra+	+	+	+	+	Tn4001-truncated II
22 (9854)	pIP1729	Tra+	+	+	+	+	Tn4001-truncated II
6 (D396)	pIP1724	Tra+	+	−	+	−	Tn4001-truncated III
14 (10619)	pIP1735	Tra+	+	−	+	−	Tn4001-truncated III
15 (10629)	pIP1736	Tra+	+	−	+	−	Tn4001-truncated III
16 (10630)	pIP1737	Tra+	+	−	+	−	Tn4001-truncated III
23 (10703)	pIP1738	Tra+	+	−	+	−	Tn4001-truncated III
11 (10642)	NAc	Tra−	−	−	+	+	Tn4001-like α
12 (10628)	NA	Tra−	−	−	+	+	Tn4001-like α
17 (10282)	NA	Tra−	−	−	+	+	Tn4001-like α
24 (10907)	NA	Tra−	−	−	+	+	Tn4001-like α

^aTra⁺, conjugative transfer; Tra⁻, nonconjugative transfer. 

^bStrain 13 carried the Gm^r marker both on pIP1734 and on a nonconjugative chromosomal Tn4001-like α structure. 

^cNA, not applicable.