FIG. 1.

Nucleotide and predicted amino acid sequence of B. bacilliformis gyrB. The nucleotide sequence of a 2,250-bp fragment containing the wild-type coumermycin A₁-sensitive B. bacilliformis gyrB is shown. Nucleotides within the 2,079-bp ORF are given in uppercase letters, and the deduced 692-residue amino acid sequence is shown below each corresponding codon. Putative consensus regulatory elements are indicated (−35, −10, ribosomal binding site [RBS]). The stop codon is marked with an asterisk. The three codons (and their corresponding amino acids) in which single nucleotide substitutions resulting in coumermycin A₁ resistance were found are boxed. The unusually long 52-residue N terminus is shown in boldface type. The predicted molecular mass of the mature protein is 77.5 kDa. The GenBank accession number for the gyrB gene is U82225.