FIG. 3.

Detection of the gyrB gene in the B. bacilliformis chromosome by DNA hybridization. (A) Ethidium bromide-stained agarose gel (1%, wt/vol) containing λ HindIII size standards (lane 1), BamHI-digested chromosomal DNA of E. coli HB101 (lane 2), BamHI-digested chromosomal DNA of B. bacilliformis KC583 (lane 3), BamHI-digested chromosomal DNA of B. bacilliformis KC584 (lane 4), no DNA (lane 5), and 2,410-bp PCR fragment containing the entire B. bacilliformis gyrB ORF. (B) The corresponding autoradiograph following DNA hybridization with the described 2,410-bp PCR fragment labeled with [³²P]dCTP. The lanes are the same as those panel A. Note the hybridization signal in both B. bacilliformis strains (lanes 3 and 4).