TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| B. bacilliformis | ||
| KC583 | Wild-type strain | 7 |
| CR1, 2, 6, 8, 9 | KC583 GyrB(Gly124→Ser) Cour | This study |
| CR4, 7, 11, 12 | KC583 GyrB(Arg184→Gln) Cour | This study |
| CR3 | KC583 GyrB(Thr214→Ala) Cour | This study |
| CR5, 10 | KC583 GyrB(Thr214→Ile) Cour | This study |
| E. coli | ||
| HB101 | Host strain used for cloning | 6, Promega |
| TOP10F′ | TOPO TA Cloning Kit host strain | Invitrogen |
| N99 | Complementation analysis strA galK | 30 |
| N4177 | Isogenic to N99 except gyrB221 (Cour) and gyrB203 (TS) | 30 |
| Plasmids | ||
| pBK-CMV | Phagemid cloning vector | Stratagene |
| pCR2.1-TOPO | Cloning vector | Invitrogen |
| pGYRB1 | pBK-CMV recombinant containing 5′ portion of B. bacilliformis gyrB in an ∼2,000-bp Sau3AI fragment; derived from λ-ZAP library | This study |
| pGYRB2 | pBK-CMV recombinant with an ∼13-kb SacI fragment containing the B. bacilliformis gyrB; derived from λ-GEM 11 library | This study |
| pGYRB3 | pCR2.1-TOPO recombinant containing entire gyrB gene in a 2,410-bp BamHI fragment; derived from TA cloning strategy | This study |