TABLE 3.
Genotypic and phenotypic analysis of B. bacilliformis gyrB mutants
| Residue | Substitution | Frequencya | MIC (μg/ml) of coumermycin A1b | Homologous GyrB lesions (reference[s]) |
|---|---|---|---|---|
| Gly124 | Ser | 41.6 | 0.2 | B. burgdorferi Gly74→Ser (40) |
| S. aureus Gly85→Ser (44) | ||||
| Arg184 | Glu | 33.3 | 0.2 | E. coli Arg136→Leu, Cys, His, Ser (11, 13) |
| B. burgdorferi Arg133→Gly, Ile (39) | ||||
| S. aureus Arg144→Ile (44) | ||||
| Haloferax sp. Arg137→His (19) | ||||
| Thr214 | Ala | 8.3 | 0.2 | B. burgdorferi Thr162→Ile (40) |
| S. aureus Thr173→Asn (44) | ||||
| Thr214 | Iso | 16.6 | 0.3 |
a
The frequency is expressed as a percentage of the 12 total isolates.
b
The MIC for wild-type KC 583 was 0.06 μg/ml. The MIC was determined for CR3, CR4, CR5, and CR9, which are representative of each of the mutant types.