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. 2023 Sep 25;8(5):e00278-23. doi: 10.1128/msphere.00278-23

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FIG 4 — vUNG interacts in complex with vPOL and vPPF. At 48 h post-transfection with the indicated expression constructs, protein lysates were DNAse treated and sonicated prior to IP and immunoblot analysis. (A) IP with anti-vUNG C1 mAb or anti-V5 mAb (B) in lysates from co-transfections with FLAG-vPOL, vPPF-V5, and vUNG-MYC. (C and D) IP with anti-FLAG mAb (C) or anti-V5 mAb (D) in lysates from co-transfections with FLAG-vPOL and vPPF-V5. (E) IP with anti-FLAG mAb in lysates from co-transfections with vUNG-MYC and FLAG-vPOL. (F) IP with anti-V5 mAb in lysates from co-transfections with vUNG-FLAG and vPPF-V5.