FIG. 4.
(A) Incorporation of [3H]CQ into hemozoin in the presence or absence of an inhibitor of plasmepsin I, Ro40-4388 (300 nM), or of cysteine proteinase, E64 (10 μM). Data represent means ± standard deviations of five separate experiments; each experiment was performed in triplicate with 2 × 109 parasitized erythrocytes. ∗∗, P < 0.05; ∗∗∗, P < 0.005. (B) Incorporation of [3H]QN into hemozoin in the presence or absence of Ro40-4388 (300 nM) or E64 (10 μM). Data represent means ± standard deviations of five separate experiments; each experiment was performed in triplicate with 2 × 109 parasitized erythrocytes. ∗∗∗∗, P < 0.001. (C) Incorporation of [3H]MQ into hemozoin in the presence or absence of Ro40-4388 (300 nM). Data represent means ± standard deviations of four separate experiments; each experiment was performed in triplicate with 3 × 109 parasitized erythrocytes. ∗∗, P < 0.05.