Figure 4. BH3 and SMAC mimetics synergize to induce selective death in senescent cells.
(A) Proliferative (top) and Doxo-induced senescent (bottom) IMR-90s were treated with ABT-263 and birinapant at the indicated concentrations for 3 d before viability was assessed relative to no drug control. Data are representative of two independent experiments performed in triplicate and are presented as mean ± s.e.m.
(B) The percent expected inhibition is subtracted from the percent observed inhibition at each combination of drug doses in Doxo-induced senescent IMR-90s to calculate drug synergy represented by excess over Bliss independence.
(C) Proliferative (top) and Doxo-induced senescent (bottom) IMR-90s were treated with ABT-199 and the SMAC mimetic birinapant at the indicated concentrations for 3 d before viability was assessed relative to no drug control. Data are representative of two independent experiments performed in triplicate and are presented as mean ± s.e.m.
(D) The percent expected inhibition is subtracted from the percent observed inhibition at each combination of drug doses in Doxo-induced senescent IMR-90s to calculate drug synergy represented by excess over Bliss independence.