TABLE 1.

Purification of VM₁ reductase from S. virginiae starting from 100 g (wet weight) of mycelia

Purification step	Total protein (mg)	Total activity (mU)	Sp act (mU/mg)	Purifica-tion factor (fold)	Yield (%)
Crude	4,850	195	0.0402	1.00	100
Ammonium sulfate	3,010	115	0.0382	0.95	59.0
DEAE-Sephacel	353	68	0.198	4.93	35.9
Sepharose 6B	187	67	0.358	8.91	34.3
HiTrap Blue	0.19	45	227	5,650	23.1