Fig. 1.

Measurement of inotodiol cytotoxicity and establishment of polyIC-induced inflammation model. (A) Chemical structure schematic diagram of inotodiol. (B) Poly(I:C) was treated at each concentration of 2.5–80 μg/ml for 24 h and cytotoxicity was measured, (C) In HDF cells, inotodiol was treated with culture media for 24 h, 1.25–80 μg/ml concentration and cytotoxicity was measured using WST-8 assay. (D) Poly(I:C) was treated for 24 h, and RNA levels of IL-1beta, IL-6, IL-8, and (E) TLR3 were analyzed by qRT-PCR after RNA isolation. Data are presented in mean values ± SD. *p < 0.05; ****p < 0.0001 vs. the control group.