Fig. 7. Exogenous H2S regulates Syvn1-mediated ubiquitination of Keap1.
A, B LC-MS/MS analysis quantifying the lysine ubiquitylome of db/db and NaHS-treated db/db mice, n = 3. C Ubiquitination levels and interaction with Nrf2 of Keap1 in cardiac tissues detected by Co-immunoprecipitation (Co-IP) assay. D The ubiquitination levels of Keap1 in HL-1 cardiomyocytes. E, F WB showing Syvn1 levels in (E) cardiac tissues, n = 3 and in (F) HL-1 cardiomyocytes, n = 3. G Potential interaction sites of Keap1 and Syvn1 predicted by HDOCK and modeled by Pymol. H The interaction between Keap1 and Syvn1 detected by Co-IP assay. All quantitative data are presented as mean ± SD from independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 by ordinary one-way ANOVA.