Table 2.
Top 40 up-and down regulated genes in Theileria annulata virulent and attenuated strain based on P value.
| Gene_name | Gene_description | Attenuated (average RPKM*) | Virulent (average RPKM*) | log2FoldChange | p value | Computed GO Functions | # TM Domains | SignalP Peptide |
|---|---|---|---|---|---|---|---|---|
| Tap370b08.q2ca38.02c | Cytochrome C oxidase subunit III (COX3 homologue), putative | 491.304754 | 165.981616 | 1.56559474 | 2.78E-07 | Cytochrome-c oxidase activity;obsolete heme-copper terminal oxidase activity | 7 | N/A |
| TA06810 | Hypothetical protein, conserved | 67.7844536 | 29.4781952 | 1.20130623 | 0.00209882 | N/A | 0 | MYSNRNITVVLLLYITHFVHS |
| Tap370b08.q2ca38.03c | Cytochrome B, putative | 339.412059 | 160.394615 | 1.08141211 | 0.00243271 | Electron transfer activity;oxidoreductase activity | 9 | N/A |
| TA07960 | Ubiquinol-cytochrome c reductase complex, subunit, putative | 90.3826372 | 45.0169167 | 1.0055784 | 0.00241293 | Ubiquinol-cytochrome-c reductase activity | 0 | N/A |
| EPrG00000717687 | rRNA | 3746.44801 | 2147.23358 | 0.80304429 | 0.00017134 | N/A | 0 | |
| TA11950 | hypothetical P-, Q-rich protein family protein, putative | 1148.83556 | 685.63827 | 0.74465277 | 0.00475935 | N/A | 1 | MINNIKYLIFVLIFRSCIFVASS |
| TA03075 | Transcription initiation factor (TBP homologue), putative | 216.967375 | 362.893299 | − 0.7420673 | 0.00151448 | DNA binding | 0 | N/A |
| TA15770 | Hypothetical protein | 120.081482 | 202.018472 | − 0.7504735 | 0.00458963 | nucleic acid binding;zinc ion Binding | 0 | N/A |
| TA09685 | Hypothetical protein, conserved | 355.15881 | 602.645021 | − 0.7628442 | 0.00106305 | Nucleic acid binding;protein binding | 0 | N/A |
| TA05280 | Hypothetical protein | 144.195729 | 249.042043 | − 0.7883609 | 0.00372746 | N/A | 4 | N/A |
| TA03875 | RNA poly(A)-binding protein, putative | 161.595485 | 280.590833 | − 0.796081 | 0.00261815 | Nucleic acid binding | 1 | N/A |
| TA15120 | Chromatin assembly protein, putative | 120.319726 | 211.979633 | − 0.8170525 | 0.00287531 | N/A | 0 | N/A |
| TA06515 | Hypothetical protein, conserved | 98.4987798 | 176.816262 | − 0.8440732 | 0.00160673 | N/A | 0 | N/A |
| TA18745 | Nicotinate-nucleotide adenylyltransferase-like protein, putative | 70.273125 | 129.167696 | − 0.8782003 | 0.00450453 | Catalytic activity;nucleotidyltransferase activity | 0 | N/A |
| TA13830 | Hypothetical protein | 53.9241059 | 99.6059114 | − 0.885301 | 0.00377777 | N/A | 0 | N/A |
| TA03785 | Proteasome subnit, putative | 141.085401 | 264.287756 | − 0.9055409 | 0.00064132 | Endopeptidase activity;threonine-type endopeptidase activity | 0 | N/A |
| TA08715 | Bacterial histone-like protein, putative | 192.899489 | 368.459133 | − 0.9336553 | 0.00225835 | DNA binding | 0 | MFTYTNSFLLLIIIICLTVES |
| TA14000 | Hypothetical protein, conserved | 161.171375 | 313.652327 | − 0.9605707 | 0.0005686 | N/A | 0 | N/A |
| TA04835 | Hypothetical protein, conserved | 40.7638947 | 82.7821973 | − 1.0220286 | 0.00206278 | N/A | 1 | N/A |
| TA09825 | Hypothetical protein, conserved | 27.3304986 | 58.0526749 | − 1.0868508 | 0.00322895 | N/A | 1 | N/A |
| TA06365 | Hypothetical protein | 40.415858 | 91.4168075 | − 1.177538 | 0.00137322 | N/A | 0 | N/A |
| TA15565 | Hypothetical protein, conserved | 40.2554646 | 92.4333837 | − 1.1992294 | 0.00083339 | N/A | 0 | N/A |
| TA12655 | Hypothetical protein | 19.7224715 | 45.3836084 | − 1.202331 | 0.00413025 | N/A | 0 | N/A |
| TA03040 | Hypothetical protein, conserved | 20.6161283 | 49.5589351 | − 1.2653718 | 0.00326846 | Nucleic acid binding | 0 | N/A |
| TA02785 | Hypothetical protein, conserved | 17.277774 | 41.8650219 | − 1.276828 | 0.00461403 | N/A | 0 | MKIILILLIINFVIN |
| TA15135 | Hypothetical protein, conserved | 22.6745348 | 56.0820538 | − 1.3064662 | 0.00295876 | Protein binding | 0 | N/A |
| TA07115 | 40S Ribosomal S12-related protein, putative | 41.1243984 | 102.839588 | − 1.3223293 | 0.00330076 | N/A | 0 | N/A |
| TA04840 | 60S Ribosomal L34 protein, putative | 307.069625 | 769.990431 | − 1.3262747 | 3.12E-08 | Structural constituent of ribosome | 0 | N/A |
| TA04425 | Ribosomal protein L18, putative | 31.6375187 | 81.5085534 | − 1.365315 | 0.00013327 | N/A | 0 | N/A |
| TA08625 | RNA polymerases I and III subunit (RPC19 homologue), putative | 18.4423666 | 48.4360839 | − 1.3930584 | 0.0015048 | DNA binding;DNA-directed 5'-3' RNA polymerase activity;protein dimerization activity | 0 | N/A |
| TA07230 | Hypothetical protein | 46.3186425 | 124.313033 | − 1.4243127 | 2.12E-05 | N/A | 1 | MKIKILFIILIINFIKC |
| TA08610 | Prefoldin subunit, putative | 26.7160243 | 78.5055381 | − 1.5550891 | 0.0004934 | Unfolded protein binding | 0 | N/A |
| TA20245 | Hypothetical protein | 18.989486 | 55.907725 | − 1.5578468 | 0.00086278 | N/A | 0 | N/A |
| TA04760 | Hypothetical protein, conserved | 27.8035159 | 90.4935596 | − 1.7025478 | 1.13E-05 | Transcription coregulator activity | 0 | N/A |
| TA20225 | Hypothetical protein | 22.2882439 | 73.9714359 | − 1.7306853 | 5.22E-05 | N/A | 1 | N/A |
| TA09695 | 30S Ribosomal protein S8, putative | 18.2494387 | 60.6542957 | − 1.7327577 | 2.17E-05 | Structural constituent of ribosome | 0 | N/A |
| TA13935 | Hypothetical protein | 21.4356829 | 76.061673 | − 1.8271553 | 1.95E-05 | N/A | 0 | N/A |
| TA15915 | Hypothetical protein, conserved | 20.0711006 | 73.5057958 | − 1.8727383 | 2.79E-05 | N/A | 0 | N/A |
| TA18140 | Hypothetical protein | 8.81402991 | 39.582718 | − 2.166997 | 9.03E-05 | N/A | 0 | N/A |
| TA08280 | Hypothetical protein | 3.22449848 | 24.2049842 | − 2.9081575 | 0.00017088 | N/A | 2 | N/A |
*RPKM = reads Per Kilobase of transcript, per Million mapped reads.