FIGURE 5.
Antioxidant profile of RSO and in vitro cell activity (A) Comparative DPPH radical scavenging (RS) for RSO and NLC-RSO. (B) Intracellular ROS levels measured by DCFDA fluorescence intensity compared to non-treated BEAS-2B cells (control group). The cells were treated with 25 μg/mL NLC-RSO, blank NP, NLC-MCT, RSO or MCT, for 24 h. Quercetin (200 µM), applied for 1 h, was used as positive control. Oxidative stress was induced by using hydrogen peroxide (H2O2) 50 µM for 1 h (n = 4). Data were expressed as mean ± standard deviation (n = 4) (*p < 0.05 **p = 0.0096 ****p < 0.0001).