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. 2023 Sep 21;3(10):2677–2691. doi: 10.1021/jacsau.3c00427

Table 1. General Summaries of H2S Measurement Approaches Using Fluorescent Probes, Methylene Blue, Monobromobimane, and Electrode Approaches.

method detection limit sample types benefits limitations
fluorescent probes probe dependent aqueous media tunable emission wavelengths selectivity, rate dependent on sensing chemistry
    biological fluids compatible with live cells and tissues signal accumulation measurements
    cell and tissue culture subcellular targeting difficult for quantification
    in vivo applications different detection methods available not reversible
methylene blue (MB) ∼2 μM aqueous media simple, high-throughput method limited detection range
    biological fluids low-cost reagents and instrumentation extracts acid-labile sulfide
    cell lysates adaptable to different applications potential bleaching by ros
      relatively short analysis time does not provide real-time measurement
monobromobimane (mBB) ∼2 nM aqueous media high sensitivity strict sample preparation and storage needs
    biological fluids separation of labile sulfide pools expensive reagents
    cell lysates stable trapped sulfide product does not provide real-time measurement
      use with complex biological samples time required for sample analysis
electrode 5–300 nM aqueous media real-time measurement frequent calibration required
    biological fluids reversible responses sensitive to solution components
    cell lysates simple experimental setup reproducibility across devices
      high sensitivity electrode lifetime