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. 2023 Oct 11;14:1279468. doi: 10.3389/fpls.2023.1279468

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Copyright © 2023 Wang, Liu, Li, Li, Zhang, Kong, Liu, Yang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FlbZIP12 overexpression enhances drought tolerance. (A) Transformation of Tartary buckwheat hairy roots. (B) Relative FlbZIP12 expression in 28-d-old hairy root lines (#2, #5, and #9) and control plants (transformed with the empty vector). (C-E) Oxidative response after 25% PEG6000 treatment. (C) SOD activity; (D) CAT activity; (E) MDA content. (F, G) Relative expression levels of stress-related genes in hairy root lines treated with 25% PEG6000 for 5 d The expression level of FlbZIP12 in the empty vector control was set to 1 (B, F, G). FlH3 was used as an internal control (B, F, G). Values are means ± SD (n = 3, B–G). Significant differences between the empty vector (EV) control and FlbZIP12-overexpressing hairy root lines are shown (*P < 0.05; **P < 0.01; one-way ANOVA with Tukey’s post-test).