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. 2023 Oct 24;20:76. doi: 10.1186/s12987-023-00479-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — ACE2⁺ cells localized next to endothelial cells in brain stem and ChP in COVID-19 patients. A-B) Representative confocal images from immunofluorescence staining of ACE2 (magenta) and von Willebrand factor (vWF, green) of brain stem (medulla oblongata) (A) and choroid plexus (B) from 3 COVID-19 patients and 1 control. Nuclei were stained with DAPI (blue). Scale bar = 20 μm. The numbers indicated in the merged image of A indicate the number of vWF⁺ vessels with ACE2 signal / total number of vWF⁺ vessels. Quantification was done from 15 images per patient