Figure 1.

Generation of a Slc38a8 mouse mutant with CRISPR-Cas9 technology. (A) Schematic representation of the Slc38a8 murine locus at chromosome 8, opposite DNA strand (UCSC Genome Browser on Mouse July 2007, NCBI37/mm9). Part of the exon 4 is expanded to show some of the amino acids encoded, including p.197Gln (equivalent to human p.200Gln, indicated by a purple square, see Supplementary Fig. S1). The DNA sequence underneath contains the sgRNA used (in bold, in black) and the PAM sequence (in bold, in red). (B) Wild-type Slc38a8 mouse protein sequence (reference) and 16 predicted proteins derived from the 16 mutated alleles identified in CRISPR genome-edited mice. The p.197Gln is indicated by a vertical red arrow. In red, erroneous amino acids generated by the corresponding mutation. An asterisk (*) identifies a newly created STOP codon. The selected FHONDA mutant allele (p.196Pro*), carried by mouse mosaic founder #A8578, is indicated by a horizontal red arrow and framed.