Figure 1.

Establishment of the G. mellonella model of Brucella infection. G. mellonella larvae were injected with 25 μl bacteria suspension, incubated at 25°C or 37°C and scored daily according to the health index scoring system. a) Representative photographs of melanization scores and cocoon scores. b) Effect of different inoculum doses of Brucella strain A19 on G. mellonella larval survival at 25°C. c) Health index scores of larvae infected with different doses of Brucella strain A19 at 25°C. d) Effect of different inoculum doses of Brucella strain A19 on G. mellonella larval survival at 37°C. e) Health index scores of larvae infected with different doses of Brucella strain A19 at 37°C. Significant differences between the Kaplan-Meier curves were identified with the log-rank test (GraphPad Prism). *P< 0.05,**P < 0.01, ***P < 0.001.