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Proteins were analyzed by size-exclusion chromatography. SEC chromatograms are shown for (A) BSA, (B) Brpt1.5, and (C) Brpt5.5 at multiple concentrations. The (D) HETP and (E) asymmetry factor (As) were calculated based on each SEC elution profile as described in the Materials and Methods section. Brpt5.5 SEC data (C) were previously published (Yarawsky et al. 2022).
