Table 2.
Shape parameters determined in the study
| M (Da) | (mL/g) | [η] | v | s 0 20,w | f/f0 | |
|---|---|---|---|---|---|---|
| BSA | 66,398.00 | 0.7325 | 3.8 | 5.2 | 4.29 | 1.3 |
| Brpt1.5 | 22,217.26 | 0.7292 | 18.1 | 24.8 | 1.54 | 1.8 |
| Brpt5.5 | 78,030.99 | 0.7280 | 75.1 | 103.2 | 2.30 | 2.7 |
| Hydration (g/g) | P | a/b (P) | β (x106) | a/b (β) | k s | ks/[η] | |
|---|---|---|---|---|---|---|---|
| BSA | 0.441 | 1.1 | 3.0 | 1.96 | 1.0 | 14.7 | 3.9 |
| Brpt1.5 | 0.516 | 1.5 | 9.3 | 2.45 | 11.7 | 23.5 | 1.3 |
| Brpt5.5 | 0.526 | 2.3 | 26.9 | 2.53 | 15.1 | 68.0 | 0.9 |
M is the theoretical molar mass based on amino acid composition.
and hydration are based on amino acid composition and reported by SEDNTERP.
For experimental values, a/b (P) was based on the Perrin function, while a/b (β) was based on the Scheraga-Mandelkern β function.
s020,w and ks values reported by SEDANAL global fitting to a non-ideal monomer model.