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. Author manuscript; available in PMC: 2023 Oct 25.
Published in final edited form as: J Pain. 2023 Mar 1;24(7):1262–1274. doi: 10.1016/j.jpain.2023.02.024

Figure 1.

Figure 1.

Graphical illustration of methods (created with BioRender.com) and timeline used in the current study. (A) Mice were separated into four groups and treated with 1.5 g/kg EtOH + 68 mg /kg pyrazole, 1.5 g/kg EtOH, 68 mg /kg pyrazole, or saline. (B) EtOH treated mice were placed into vapor chambers and exposed to 16 hours of EtOH vapor followed by a period of 8 hours of ambient room air. AC mice were also placed into a different vapor chamber without the presence of EtOH. (C) Four mice from each treatment group were used for either hotplate or von Frey behavioral testing. (D) Schematic representation of the EtOH exposure and behavioral timeline. Mice underwent 5 weeks of CIEV and withdrawal.