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. Author manuscript; available in PMC: 2023 Oct 25.
Published in final edited form as: Mol Cell. 2022 Jun 24;82(16):3103–3118.e8. doi: 10.1016/j.molcel.2022.06.001

Figure 4. ML-optimized DAISY barcodes have robust performance across cell lines with doxycycline-controllable tunability.

Figure 4.

(A) Comparison of barcode entropy demonstrating consistent performance of CLOVER-optimized DAISY barcodes in A375 melanoma and A549 lung adenocarcinoma cell lines. Top barcodes used in later experiments are highlighted. (B) Comparison of total barcode entropy across all clones within each indicated cell type. (C) Consistent indel mutation length distributions of editing outcomes within the DAISY barcode (bc859) across cell lines (D) Experiment design to measure doxycycline-dependent tunability of top DAISY barcodes in A375 cells. Low and High-dox were 40 and 1000 ng/mL. (E) Change in the barcode entropy over time using low and high-dox. (F) Rate kinetics of barcode entropy (based on the Exponential plateau model) across doxycycline dosages and biological replicates.