Fig. 3. Michaelis–Menten kinetic analysis for SLA dehydrogenase using NAD⁺, D-SLA, NADP⁺ and GAP as substrates. (a and b) Michaelis–Menten and Lineweaver–Burk (inset) plots for RlGabD under pseudo first-order conditions of [D-SLA] = 0.25 mM (for panel a) and [NAD⁺] = 0.25 mM (for panel b). (c and d) Michaelis–Menten and Lineweaver–Burk (inset) plots for RlGabD under pseudo first-order conditions of [NADP⁺] = 0.25 mM (for panel c) and [D-SLA] = 0.25 mM (for panel d). (e and f) Michaelis–Menten and Lineweaver–Burk (inset) plots for oxidation of GAP by RlGabD under pseudo first-order conditions of [NAD⁺] = 0.25 mM (for panel e) and [NADP⁺] = 0.25 mM (for panel f).