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. 2023 Oct 4;622(7984):834–841. doi: 10.1038/s41586-023-06611-6

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, b, Expression of the indicated genes in primary cultured chondrocytes of mouse fetal growth plates upon conditional knockout of Hif-1α in either 20% O₂ (a) or 1% O₂ for 6 h (b). n = 3 biologically independent samples. c, d, Expression of the indicated genes in primary cultured chondrocytes of mouse fetal growth plates upon conditional knockout of Hif-2α in either 20% O₂ (c) or 1% O₂ for 6 h (d). n = 3 biologically independent samples. e, f, Expression of the indicated genes in primary cultured chondrocytes of mouse fetal growth plates upon conditional knockout of both Hif-1α and Hif-2α (Hif-1/2α) in either 20% O₂ (e) or 1% O₂ for 6 h (f). n = 3 biologically independent samples. g, Quantification of Kdm5a protein by Western blot analysis of total protein lysate extracted from Ctrl or si-Kdm5a primary cultured chondrocytes. A representative Western blot is shown on the left, and quantification of all biological replicates is provided on the right. Data were normalized to α-tubulin (n = 3 biologically independent experiments). For gel source data, see Supplementary Fig. 1k. h, Quantification of Kdm5b protein by Western blot analysis of total protein lysate extracted from Ctrl or si-Kdm5b primary cultured chondrocytes. A representative Western blot is shown on the left, and quantification of all biological replicates is provided on the right. Data were normalized to α-tubulin (n = 3 biologically independent experiments). For gel source data, see Supplementary Fig. 1l. i, j, Expression of the indicated genes in primary cultured chondrocytes upon knockdown of Kdm5a or Kdm5b in either 20% (i) or 1% O₂ (j) for 6 h. n = 3 biologically independent experiments. k, l, ChIP-qPCR analysis results of H3K4me3 for the indicated genes in primary cultured chondrocytes siRNA depleted of Kdm5a in either 20% (k) or 1% O₂ (l). The data are mean with SEM of triplicate experiments. P values were calculated using two-tailed Student’s t-test (a-f) or one-way ANOVA tests (g-l). The exact P-values of comparison are presented in the figures, respectively.

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